image   Products for Metabolic Profiling and Standards

Isotopic Ratio Outlier Analysis (IROA®) Kits 

The golden rule for accurate and reproducible metabolic profiling is to have an internal standard for every metabolite measured.

 HERE'S HOW:

  1. IROA® Quantitation Kits (make your own internal standards that exactly mirror the metabolome you wish to measure) - contain all of the reagents necessary for the successful labeling, quantitation and identification of biochemical compounds. ClusterFinder software tool and statistical analysis tools are provided with any kit purchase
    • IROA 100 - to create yeast Internal Standards, identify and quantitate yeast metabolites
    • IROA 200 - to create bacterial Internal Standards, identify and quantitate bacterial metabolites
    • IROA 300 - to create mammalian Internal Standards, identify and quanitate mammalian metabolites
  2. IROA® Internal Standard Kits - contain pre-labeled complex internal standards for the identification of 100's of metabolites across time and instruments. ClusterFinder software tool and statistical analysis tools are provided with any kit purchase.
    • IROA® Workflow -  Cost effective identification and measurement of 100’s of analytes using unique complex stably-labeled (U- 95%13C) yeast IROA Internal Standard (IROA-IS)  
    • NOTE: For situations where it is not practical or possible to label experimental samples in cell culture systems such as biopsies, field-grown plants or large-scale fermentation, please refer to the IROA® Phenotypic Experimental Protocol section.

      To determine the flux of a specific precusor through metabolic pathways, the IROA® Fluxomic QuantitationKit may be used.